Particle length,
Host range,
Capsid symmetry,
Antigenic properties, and
DNA conformation and ratios of nucleotides per subunit.
|
Type Species |
(M13) |
Infectivity is sensitive to sonication; ether sensitivity is variable. Nucleic acid is 6-14% by weight of particle, and G+C is 40-60%. Sedimentation rates are 42 ± 2S. Virions have no carbohydrate. Host range is certain genera in the gamma-purple phylogenetic branch of Gram-negative bacteria; i.e., Enterobacteria, Pseudomonas, Vibrio, and Xanthomonas.
List of Species Demarcation Criteria in the Genus
Demarcation criteria in the genus are:
|
Particle length, |
|
Host range, |
|
Capsid symmetry, |
|
Antigenic properties, and |
|
DNA conformation and ratios of nucleotides per subunit. |
An individual species in this genus is distinguishable by its host range, major coat protein sequence, and capsid symmetry. For example, the species Ff includes strains fd, f1, and M13 which have the same host range, capsid symmetry, and coat sequence (except for a single amino acid; their DNA sequences differ less than ~ 1.5%). Although mutations, natural or otherwise, might dramatically affect virion length, ratios of nucleotides to subunits, DNA conformation, and antigenic properties of these strains (as in phage display work), species boundaries are not likely to be crossed. The viruses If1, IKe, and I2-2 have the same capsid symmetry and very similar sequences, but their host ranges differ, so they are different species.
Official virus species names are in italics. Tentative virus species names, alternative names ( ), strains or serotypes are not italicized. Virus names, their host ranges { }, genome sizes (in kb), genome sequence accession numbers [ ], and assigned abbreviations ( ) are:
|
1-Phages of Enterobacteriaceae: | ||||
|
Enterobacteria phage If1 |
{E. coli, S. typhimurium; IncI} |
9 kb |
(If1)* | |
|
Enterobacteria phage IKe |
{E. coli; Inc I2, N, P-1} |
6.9 kb |
[X02139] |
(IKe)* |
|
Enterobacteria phage I2-2 |
{E. coli; Inc I2} |
6.7 kb |
[X14336] |
(I2-2)* |
|
Enterobacteria phage M13 |
{E. coli IncF, Hfr} |
6.4 kb |
[V00604] |
(M13, Ff)* |
|
Enterobacteria phage f1 |
{E. coli IncF, Hfr} |
6.4 kb |
[J02448] |
(f1,Ff)* |
|
Enterobacteria phage fd |
{E. coli IncF, Hfr} |
6.4 kb |
[V00602] |
(fd,Ff)* |
|
Enterobacteria phage AE2 |
{E. coli IncF, Hfr} |
(AE2) | ||
|
Enterobacteria phage |
(probably all Ff) | |||
|
Enterobacteria phage Ec9 |
||||
|
Enterobacteria phage HR |
||||
|
Enterobacteria phage ZJ/2 |
||||
|
Enterobacteria phage X-2 |
{E. coli, S. typhi, Sr. marc.; IncX, unique R775} |
(X-2) | ||
|
Enterobacteria phage C-2 |
{E. coli, S. typhi; IncC} |
8.1 kb |
(C-2) | |
|
Enterobacteria phage X |
{E. coli, S. typhi, Sr. marc., others; IncX, I2, N, P-1, others} |
(X) | ||
|
Enterobacteria phage PR64FS |
{E. coli; IncR} |
(PR64FS) | ||
|
Enterobacteria phage SF |
{E. coli, K. pneumoniae, S. typhi, others; IncS} |
(SF) | ||
|
Enterobacteria phage tf-1 |
{E. coli, S. typhi; IncT} |
(tf-1) | ||
|
2- Phages of Spirillaceae: | ||||
|
Vibrio phage 493 |
{V. cholera 0139-Aj27, El Tor} |
9.3 kb |
(493) | |
|
Vibrio phage fs1 |
{V. cholera 01, 0139} |
6.3 kb |
[D89074] |
(fs1) |
|
Vibrio phage fs2 |
{V. cholera 01} |
8.5 kb |
(fs2) | |
|
Vibrio phage CTX |
{V. cholera 0395, Peru 15, El Tor, others; lysogenic} |
7 kb |
[U83795-6] |
(CTX) |
|
Vibrio phage v6 |
{V. parahaemolyticus} |
(v6) | ||
|
Vibrio phage Vf12 |
(Vf12) | |||
|
Vibrio phage Vf33 |
{V. parahaemolyticus} |
8.4 kb |
(Vf33) | |
|
Vibrio phage VSK |
{V. cholera 0139-B04, lysogenic V. cholera 0139-P07, normal} |
(VSK) | ||
|
3-Phages of Pseudomonadaceae: | ||||
|
Pseudomonas phage Pf1 |
{P. aeruginosa PAK} |
7.3 kb |
[X52107] |
(Pf1)* |
|
Pseudomonas phage Pf2 |
{P. aeruginosa PAK} |
(Pf2) | ||
|
Pseudomonas phage Pf3 |
{P. aeruginosa PAO; IncP-1} |
5.8 kb |
[M11912] |
(Pf3)* |
|
4-Phages of Xanthomonadaceae: | ||||
|
Xanthomonas phage Cf1c |
{X. camp. citri : normal} |
7.3 kb |
[M57538,U41819] |
(Cf1c) |
|
Xanthomonas phage Cf1t |
{X. camp. citri : lysogenic} |
[U08370] |
(Cf1t) | |
|
Xanthomonas phage Cf16 |
{X. camp. citri : neolysogenic} |
(Cf16) | ||
|
Xanthomonas phage Cf1tv |
{X. camp. citri -Cf1t : lytic} |
(Cf1tv) | ||
|
Xanthomonas phage Lf |
{X. campestris pv. campestris : lysogenic} |
6.0 kb |
[X70327-31, U10884, U38235, AF018286] |
(Lf) |
|
Xanthomonas phage Xf |
{X. campestris pv. oryzea} |
7.4 kb |
(Xf)* | |
|
Xanthomonas phage Xfo |
{X. oryzae pv. oryzea} |
7.6 kb |
(Xfo) | |
|
Xanthomonas phage Xfv |
{X. campestris pv. vesicatoria} |
6.8 kb |
(Xfv) | |
* An asterisk after the abbreviation in bold indicates that capsid symmetry has been assigned; for those in group 1 it is C5S2; for those in groups 2 and 4 it is C1S5.4. E. coli: Escherichia coli, S. typhi: Salmonella typhi, Sr. marc.: Serratia marcescens, K. pneumoniae: Klebsiella pneumoniae, V. cholera: Vibrio cholera, V. parahaemolyticus: Vibrio parahaemolyticus, P. aeruginosa: Pseudomonas aeruginosa, X. camp. citri: Xanthomonas campestris citri, X. oryzae: Xanthomonas oryzae.
Tentative Species in the Genus
None.
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|  |
A