Figure 1 (Left) Section of palisade mesophyll cell from a leaf of Nicotiana clevelandii systemically infected with Carrot mottle virus (CMoV), showing enveloped structures (E) about 52 nm in diameter in the cell vacuole (V) in association with the tonoplast (T). The bar represents 250 nm. (Right) Enveloped structures about 52 nm in diameter in a partially purified preparation from CMoV-infected N. clevelandii, stained with 2% uranyl acetate. The bar represents 100 nm.
Figure 2 Diagram showing the arrangement of ORFs in the genomic RNA molecule of Groundnut rosette virus (GRV). The continuous horizontal line represents the genome RNA, and the numbered blocks the correspondingly numbered ORFs. The lower part of the diagram shows the predicted translation products, with their Mr values. The potential product of ORF1 has not been shown to exist. The single product produced from ORFs 1 and 2, probably as a result of a -1 frameshift event at the point marked FS, is thought to be a polymerase because it contains, in the ORF2 region, sequences characteristic of viral RNA-dependent RNA polymerases. The function of the ORF3 product is unknown. The ORF4 product, marked MP, has a cell-to-cell movement function.
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