Figure 1 Negative contrast electron micrograph of isometric particles of Cricket paralysis virus (CrPV). The bar represents 100 nm. (Courtesy of C. Reinganum.)
Figure 2 Genome structure of Drosophila C virus (DCV). The approximate positions of the putative helicase (H), protease (P) and replicase (Rep) in the non-structural proteins are shown. The structural proteins are encoded by the 3 ORF and the translation product is processed into at least three (and in some members four) mature capsid proteins. In the case of Plautia stali intestine virus (PSIV), it is the second of the three capsid proteins that it further processed to produce the fourth capsid protein.
Figure 3 Unrooted phenogram showing the relationships of the “CrPV-like viruses” (shaded on the phenogram) to representatives of the families Picornaviridae, Comoviridae, Sequiviridae and the unassigned insect viruses; Infectious flacherie virus (IFV) and Acyrthosiphon pisum virus (APV). The phenogram was constructed from an amino acid similarity matrix of the polymerase region using the neighbor-joining method and the MEGA software. A boostrap analysis has been performed and the values are indicated at the branching points. Viruses included in the analysis, abbreviation ( ) and accession numbers [ ] are: “CrPV-like viruses”; Drosophila C virus (DCV) [AF014388], Himetobi P virus (HiPV) [AB017037], Plautia stali intestine virus (PSIV) [AB006531], Rhopalosiphum padi virus (RhPV) [AF022937], Comoviridae; Cowpea severe mosaic virus (CPSMV) [M83830], Picornaviridae; Aichi virus (AiV) [AB010145], Encephalomyocarditis virus (EMCV) [M81861], Equine rhinitis B virus (ERBV) [X96871], Foot and mouth disease virus (FMDV) [Foot and mouth disease virus O: X00871], Hepatitis A virus (HAV) [Human hepatitis A virus: M14707], Human rhinovirus B virus (HRV-B) [HRV-14: X01087], Poliovirus (PV) [Human poliovirus 1: J02281], Sequiviridae; Parsnip yellow fleck virus (PYFV) [D14066], Rice tungro spherical virus (RTSV) [M95497], Unassigned insect viruses; Acyrthosiphon pisum virus (APV) [AF14514], Infectious flacherie virus (IFV) [AB000906]. Branch lengths are drawn to scale.
Figure 4 Unrooted phenogram showing the relationships amongst members of the CrPV-like viruses genus constructed from an amino acid similarity matrix of the CP1 using the neighbor-joining method of the MEGA software. Viruses included in the analysis, abbreviation ( ) and accession numbers [ ] are: Cricket paralysis virus (CrPV) [M21938], Drosophila C virus (DCV) [AF014388], Himetobi P virus (HiPV) [AB017037], Plautia stali intestine virus (PSIV) [AB006531], Rhopalosiphum padi virus (RhPV) [AF022937]. Branch lengths are drawn to scale.
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