Figure 1 (Left) Diagrammatic representation of arenavirus virion structure. L, L protein (RNA polymerase); NC, nucleocapsid; R, ribosome. (Adapted from Andrew Featherstone and Christopher Clegg.) (Right) Negative contrast electron micrographs of particles of Lymphocytic choriomeningitis virus (LCMV). A. Thin section showing several virions budding from the surface of an infected BHK-21 cell. B-D. Cryo-electron microscopic images of purified unstained virions frozen in vitreous ice, taken at -1.5, -3 and -4 microns defocus. Arrowheads indicate glycoprotein spikes which are composed of a transmembrane GP2 and globular GP1 head arranged in a tetrameric configuration. The bars represent 100 nm. (R. Milligan, J. Burns and M. Buchmeier, Persistent Viral Infections © by John Wiley & Sons, Ltd. Reproduced with permission.)
Figure 2 Organization, transcription and replication of the arenavirus L and S RNAs. Regions encoding the L, Z, GPC and N proteins are shown as shaded boxes. The intergenic regions separating the ORFs are indicated by gray boxes. The sgRNAs which function as messengers are indicated. (From V. Romanowski).
Figure 3 Phylogenetic relationships among the members of the family Arenaviridae. Partial N gene nucleotide sequences corresponding to nts 1770-2418 of Tacaribe virus S RNA sequence (M20304) were aligned (PILEUP, adjusted manually) and analyzed by maximum parsimony using PAUP (Courtesy of M. Bowen, C.J. Peters, S. Nichol.)
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