Figure 1 Electron micrographs of sucrose density gradient purified RNPs of Rice hoja blanca virus (RHBV). (Left) Small circular RNPs from the slowest sedimenting RHBV RNP. (Right) Larger, circular RNPs from the fastest sedimenting RHBV RNP. The bar represents 100 nm. (Courtesy of A.M. Espinoza.)
Figure 2 Genome organization of Maize stripe virus (MSpV). Boxes indicate the positions and designations of the ORF translation products. (V) Signifies virus-sense RNA and (VC) signifies virus-complementary sense RNA.
Figure 3 Diagram of the expression of the ambisense RNA of tenuiviruses. The black circle signifies the Cap, the broken line signifies non-viral nucleotides, V signifies virus-sense RNA and VC signifies virus-complementary sense RNA.
Figure 4 Most parsimonious phylogenetic tree of tenuiviruses (MStV, RGSV, RHBV and RSV), phleboviruses (PUTV, RVFV and UUKV) and a tospovirus (TSWV). The bunyavirus BUNV was used as outgroup virus. Input data were the combined matrix of nucleocapsid proteins and virion membrane glycoproteins or membrane glycoproteins-like proteins, each of which was aligned using CLUSTAL V. Gaps were treated as new character states in the tree search. Figures indicate bootstrap probabilities for 1000 replicas. Accession numbers for sequence data of the nucleocapsids are as follows: MStV, M57426; RGSV, AB000403; RHBV, L07940; RSV, X53563; PUTV, K02736; RVFV, X53771; TSWV, D00645; UUKV, 33551; and BUNV, D00353. Accession numbers for sequence data of the virion membrane glycoproteins and related proteins are as follows: MStV, U53224; RGSV, AB010376; RHBV, L54073; RSV, D13176; PUTV, M11156; RVFV, M11157; TSWV, S48091; UUKV, M17417; and BUNV, M11852.
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