-N-4a-4b-M-G-L-5. N represents a Mr 51 
103 nucleoprotein. The intergenic regions contain highly conserved consensus sequences. The 5-non-coding trailer sequence of 187 nts has extensive complementarity to the 3-leader.
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Type Species |
(LNYV) |
Two genera of plant rhabdoviruses have been established. The viruses are primarily distinguished on the basis of the sites of virus maturation (cytoplasm: Cytorhabdovirus; nucleus: Nucleorhabdovirus). However, exceptions exist and the significance of this property is not known. The interrelationships of the different plant viruses within or between the two genera or with the unassigned plant viruses have yet to be established at the genetic level. There is no significant sequence similarity between analogous genes of the three species analyzed to date. A wide variety of plants are susceptible to plant rhabdoviruses although each virus usually has a restricted host range. Most of the plant rhabdoviruses are transmitted by leafhoppers, planthoppers, or aphids, although mite- and lacebug-transmitted viruses (one each) have also been identified. Some viruses are transmitted in contaminated sap. In all carefully examined cases, viruses have been shown to replicate in the insect vector as well as in the plant host.
Cytorhabdoviruses replicate in the cytoplasm of infected cells in association with masses of thread-like structures (viroplasms). Virus morphogenesis occurs in association with vesicles of the endoplasmic reticulum. A nuclear phase has been suggested but not proven in the replication of some cytorhabdoviruses, e.g., Lettuce necrotic yellows virus (LNYV). Evidence of the nuclear involvement in the replication of others is lacking (e.g., Barley yellow striate mosaic virus, BYSMV). Endogenous transcriptase activity is readily detectable in virus preparations. The genome organization of LNYV is similar to that of Sonchus yellow net virus (SYNV) (see nucleorhabdoviruses). Preceded by a non-coding 84 nts leader sequence, the gene order is 3-N-4a-4b-M-G-L-5. N represents a Mr 51 103 nucleoprotein. The intergenic regions contain highly conserved consensus sequences. The 5-non-coding trailer sequence of 187 nts has extensive complementarity to the 3-leader.
List of Species Demarcation Criteria in the Genus
In the genus Cytorhabdovirus, species are primarily differentiated by host range and vector specificity. Nucleic acid hybridization has been used to provide confirmation of species and serological criteria have enabled verification of common species that infect different hosts. However, no virus strains have been defined unambiguously using serology. Only partial sequences are available for LNYV. Thus this criterion is not presently sufficient for discrimination of species. Hybridization using cloned probes has been used to verify species within the genus and these analyses should be emphasized in future studies.
Official virus species names are in italics. Tentative virus species names, alternative names ( ), strains or serotypes are not italicized. Virus names, vector type { }, CMI/AAB description numbers ( ), genome sequence accession numbers [ ], and assigned abbreviations ( ) are:
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Barley yellow striate mosaic virus |
{leafhopper} (312) |
(BYSMV) | |
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Broccoli necrotic yellows virus |
{aphid} (85) |
(BNYV) | |
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Festuca leaf streak virus |
(FLSV) | ||
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Lettuce necrotic yellows virus |
{aphid} (26, 243) |
[L24364, L24365, L30103] |
(LNYV) |
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Northern cereal mosaic virus |
{leafhopper} (322) |
(NCMV) | |
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Sonchus virus |
(SonV) | ||
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Strawberry crinkle virus |
{aphid} (163) |
(SCV) | |
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Wheat American striate mosaic virus |
{leafhopper} (99) |
(WASMV) |
Tentative Species in the Genus
None reported.
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