Figure 1 (Upper) Scheme of virion in cross-section; (Lower left) Negative contrast electron micrograph of a Marburg virus (MARV) preparation purified and concentrated by centrifugation from guinea pig serum, 7 days after intraperitoneal infection, stained with 1% phosphotungstate, 20,000; (Lower right) Negative contrast electron micrograph of a Zaire Ebola virus (ZEBOV) preparation, Vero cell culture supernatant, 4 days after infection. The bars represent 200 nm. (Courtesy of T.W. Geisbert.)
Figure 2 Diagram of filovirus genome organization. The genes which encode the structural proteins are identified in the genomes and are drawn to scale. Shaded squares designate coding regions and dark lines non-coding regions. Genes begin with a conserved transcriptional start and end with a transcriptional stop site (polyadenylation site). Adjoining genes are either separated from one another by an intergenic region (short arrowheads) or overlap. The editing site on the GP gene of Ebola viruses is indicated by a black triangle. At the extreme 3 and 5 ends of the genomes are leader and trailer sequences, respectively, that are in part complementary.
Figure 3 Phylogenetic tree showing the relationships between the glycoproteins genes of filoviruses. The entire coding region for the glycoprotein gene of the viruses shown was used in maximum parsimony analysis, and a single most parsimonious tree was obtained. Numbers indicate bootstrap confidence values for branch points. Maximum parsimony analyses were performed by using the entire coding regions of the GP genes of the characterized filoviruses (Sanchez, Trappier, Mahy, Peters and Nichol, 1996). The Phylogenetic Analysis Using Parsimony version 3.1.1 software, using the branch-and-bound search option. A 4:1 transversion:transition weighting was used in analyses. A single most parsimonious tree was obtained, and bootstrap analysis strongly supports a common evolutionary origin for the viruses, early separation of evolution of Marburg virus and Ebola viruses and substantial differences between four species of Ebola-like viruses.
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