Table Gallery

Table 1 Enterobacteria phage PRD1 (PRD1) proteins and their functions.


Phage protein^a/>	Protein gene	Mutant^b	10³^c	Function	Location

P1	I	+	63.3	DNA polymerase^f	host cell cytoplasm
P2	II	+	63.7	adsorption protein	capsid (5-fold symmetry position)
(spike complex)
P3	III	+	43.1	major capsid protein	capsid
P5	V	-	34.3	spike complex	capsid (5-fold symmetry position)
P6	VI	-	17.6	?	capsid
P7	VII	+	27.1	infectivity	viral membrane, surface
P8	VIII	+	29.5	protein primer	genome terminal protein
P9	IX	+	25.8	DNA packaging	capsid
					(ATPase)
P10	X	+	20.6	assembly factor^f	host cell membrane
P11	XI	+	18.2	infectivity,	viral membrane, surface
					aggregation factor
P12	XII	+	16.6	ssDNA-binding^f	host cell cytoplasm
P14	XIV	+	17^e	infectivity	viral membrane, surface
					(fragment of P7)
P15	XV	+	17.3	lytic muramidase	host cell cytoplasm
P16	?^d	+	17^e	infectivity	viral membrane
P17	XVII	+	9.5	assembly factor^f	host cell cytoplasm
P18	XVIII	+	9.8	infectivity	viral membrane, integral
(ORFm)
P19	XIX	+	10.5	ssDNA-binding^f	host cell cytoplasm
P20	XX	+	4.7	DNA packaging	viral membrane, integral
(ORFj)				(stabilization)
P22	XXII	+	5.5	DNA packaging	viral membrane, integral
(ORFk)				(stabilization)
P30	XXX	+	9.2	capsid stabilizing	capsid
(ORFp)				(cementing) protein
P31	XXXI	+	13.7	spike complex	capsid (5-fold symmetry position)
(ORFc)

^a Note that there are additional proteins as deduced from sequence analysis for which mutant isolation is missing and thus their function remains obscure. These have been omitted from this table.

^b Availability of a PRD1 nonsense mutant is indicated by a plus sign.

^c Molecular ratio calculated from the sequence (Bamford et al., 1991).

^d None of the separately cloned PRD1 genes was able to complement the class XVI nonsense mutant, thus the gene encoding this protein is still not localized.

^e Molecular mass deduced from SDS-polyacrylamide gel.

^f Nonstructural protein.