DESCRIPTION OF VIRUSES

Family Myoviridae

Genus “SPO1-like viruses”

Genus “SPO1-like viruses”

Type Species

Bacillus phage SPO1

(SPO1)

Distinguishing Features

Phage heads show conspicuous capsomers. DNA is terminally redundant, contains 5-hydroxymethyluracil, and codes for a type A (E. coli Pol I) DNA polymerase.

Virion Properties

Morphology

Virions have icosahedral heads of about 94  nm in diameter with conspicuous capsomers. Tails measure 150 18  nm and have a small collar and a 60  nm wide base plate.

Physicochemical and Physical Properties

Virion Mr is 180 106; buoyant density in CsC1 is 1.54  g/cm3; S20w is 794S.

Nucleic Acid

Genomes are about 140-160  kbp in size, and have a G+C content of 42%. Thymine is replaced by 5-hydroxymethyluracil.

Proteins

Virions contain 53 proteins (16 in the head and 28 in the base plate). Type A DNA polymerase is encoded in the phage genome.

Lipids

Not known.

Carbohydrates

Not known.

Genome Organization and Replication

The genetic map is linear and may comprise as many as 190-200 genes. Related functions cluster together. The genome has a terminally redundancy of about 12  kbp. After infection, host syntheses are shut off and replication starts at two sites. Phage-encoded sigma factors are used to modify and appropriate host RNA polymerases for phage syntheses.

Biological Properties

Phages are virulent and are specific for bacilli. Distribution is worldwide.

List of Species Demarcation Criteria in the Genus

Not applicable.

List of Species in the Genus

Official virus species names are in italics. Tentative species names, alternative names ( ), strains or serotypes are not italicized. Virus names and assigned abbreviations ( ) are:

Species in the Genus

Bacillus phage SPO1

(SP01)

Bacillus phage SP8

(SP8)

Bacillus phage SP82

(SP82)

Tentative Species in the Genus

Bacillus phage AR1

(AR1)

Bacillus phage GS1

(GS1)

Bacillus phage I9

(I9)

Bacillus phage NLP-1

(NLP-1)

Bacillus phage SP5

(SP5)

Bacillus phage SW

(SW)

Bacillus phage e

(e)

Bacillus phage 25

(25)

Bacillus phage 2C

(2C)

Lactobacillus phage 222a

(222a)


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